Recombinant DNA technology, tools, processes.
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Q1 · Biotechnology — Principles and Processes · EASY
Which enzyme is commonly used to cut DNA at specific recognition sequences in recombinant DNA technology?
Q2 · Biotechnology — Principles and Processes · MEDIUM
In a recombinant DNA experiment, a plasmid vector is cut with EcoRI restriction enzyme producing sticky ends with the sequence 5'-AATT-3'. Which of the following DNA fragments can be ligated to this plasmid?
Q3 · Biotechnology — Principles and Processes · EASY
Which of the following is the correct sequence of steps in recombinant DNA technology?
Q4 · Biotechnology — Principles and Processes · MEDIUM
A researcher wants to clone a 8 kb gene fragment. Which vector would be most appropriate for this purpose?
Q5 · Biotechnology — Principles and Processes · HARD
In the polymerase chain reaction (PCR), if the initial amount of target DNA is 50 ng and the reaction runs for 30 cycles with 100% efficiency, what will be the approximate amount of amplified product? (Assume each cycle doubles the DNA)